L. Razeghi; M. Azizi; S.M. Ziaratnia; A.R. Bagheri; S.H. Nemati
Abstract
K. odoratissima Mozaff., belonging to the Apiaceae family, is a known medicinal and forage species, endemic to Iran. It has not been reported in other regions of the world. Due to the excessive harvesting in the early period of growth and the relatively high time required for the establishment and seed ...
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K. odoratissima Mozaff., belonging to the Apiaceae family, is a known medicinal and forage species, endemic to Iran. It has not been reported in other regions of the world. Due to the excessive harvesting in the early period of growth and the relatively high time required for the establishment and seed production, this species does not have the opportunity to revitalize and seed production and for this reason, it is considered as one of the endangered species of Iran. Advances in tissue culture can be most effective in propagation of endangered plants as well as increasing the genetic potential of medicinal plants. To study the effect hormonal treatments on callus induction sterile seedling explants were prepared. Treatments included MS basal medium along with different hormonal levels (2,4-D (0.5, 1 and 2 mg/l) or a1, a2, a3 + Kin (0, 0.5 and 1 mg/l) or b1, b2, b3) and Ms basal medium with (NAA (0.5, 1 and 2 mg/l) or a4, a5, a6 + BA (0, 0.5 and 1 mg/l) or b4, b5, b6). After a month, growth (size), weight and callus induction percentage were measured and compared. Considering the above traits, the maximum size of callus (6.41mm) and the most callus induction percentage (93%) were obtained at 2,4-D (2mgl-1)+kin (0.5mgl-1) while the highest callus weight was obtained at NAA (1mgl-1) + BA (0.5mgl-1).Combined hormones (2mg/l 2,4-D + 0.5 mg/l kin) and (2mg/l NAA + 0.5mg/l BA) in two media, MS and B5, along with three antioxidants (PVP, PVPP and PVP + PVPP), totally 12 treatments, were evaluated within four consecutive weeks under broth condition and cell suspension establishment. According to the results, significant differences were found (p< 0.05) among the factors affecting cell dry weight while these factors had no significant effect on fresh weight. The highest fresh and dry weights were obtained at B5 medium with PVP (0.1) (1.6940 gr). Fresh and dry weight of cells showed an increasing trend during the consecutive weeks.
A. Farzaneh; M.T. Ebadi; S.H. Nemati; H. Arouiee
Abstract
In order to investigate the germination factors of two improved cultivars and one Iranian landrace of cornflower (Centaurea cyanus) under salt stress conditions a factorial experiment based on completely randomized design with two factors in three replications was conducted at experimental laboratory ...
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In order to investigate the germination factors of two improved cultivars and one Iranian landrace of cornflower (Centaurea cyanus) under salt stress conditions a factorial experiment based on completely randomized design with two factors in three replications was conducted at experimental laboratory of department of horticulture, Ferdowsi University of Mashhad during 2009. The main factor included two improved cultivars of cornflower (Ball blue and Ball junge) and one Iranian landrace and second factor included seven salinity levels (0, 50, 100, 150, 200, 250 and 300 milli molar NaCl). Measured characteristics were germination percentage, germination velocity, vigor index, radicle and plumule length. According to the results of ANOVA, different levels of salt stress had significant effects on all measured characteristics while the effect of cultivar type was only significant on germination percentage, germination velocity and vigor index. The interaction between salinity and cultivar type had significant effect on all measured characteristics. The results showed that with increase in salinity level, all measured characteristics were significantly decreased. The most germination percentage and germination velocity (51.04 percent and 11.82 seed/day respectively) were obtained from Ball blue in control treatment and the lowest (9.32 percent and 0.5 seed/day respectively) was obtained from Iranian landrace and Ball junge cultivar in 300 mili molar NaCl. Decrease of plumule length was more than that of radicle length under salinity stress. In this study, improved cultivars of cornflower and Iranian landrace showed different responses to salt stress due to the diversity in their genotypes. Generally, Ball blue was identified as the most salt tolerance cultivar in germination stage.
